![]() ![]() CCD cameras also offer convenience by providing a digital record of experiments for data analysis, sharing, and archiving, and by eliminating the need to continually purchase consumables for film development. Bio-Rad's Clarity Western ECL Substrate is compatible with any HRP-conjugate secondary detection reagent and ideal for both digital and film-based imaging.ĬCD cameras have a linear response over a broad dynamic range - 2–5 orders of magnitude - depending on the bit depth of the system. Additionally, quantitation of data collected by exposure to film requires digitization (that is, scanning of X-ray film with a densitometer). ![]() A process termed preflashing can improve linearity, but this requires extra equipment and effort. To gather information from a blot that has both intense and weak signals, multiple exposures are required to produce data for all samples in the linear range of the film. Film is a sensitive medium for capturing the chemiluminescent signal but suffers from a sigmoidal response to light with a narrow region of linear response, which limits its dynamic range. Traditionally, the chemiluminescent signal from blots was detected by X-ray film. Decreasing costs, superior quantitative data quality, and better ease of use are making CCD cameras and imagers preferred over film for these detection techniquesĬomparison of western blot documentation and analysis methods.įor chemiluminescence detection, CCD imaging is the easiest, most accurate, and fastest method. This method also requires the investment in and maintenance of a film developer and often requires processing multiple film sheets to obtain a usable image. ![]()
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